Fluorogenic cell surface glycan labelling with fluorescence molecular rotor dyes and nucleic acid stains
A. Koçak, A.K. Homer, A. Feida, F. Telschow, J.L.Gorenflos López, C. Baydaroglu, M. Gradzielski, C.P.R. Hackenberger, U. Alexiev, O. Seitz – 2024
We show that covalent labelling of sialic acids on live cell surfaces or mucin increases the fluorescence of the fluorescence molecular rotors (FMRs) CCVJ, Cy3 and thioazole orange, enabling wash-free imaging of cell surfaces. Dual labelling with an FMR and an environmentally insensitive dye allows detection of changes that occur, for example, when cross-linking is altered.
Title
Fluorogenic cell surface glycan labelling with fluorescence molecular rotor dyes and nucleic acid stains
Author
A. Koçak, A.K. Homer, A. Feida, F. Telschow, J.L.Gorenflos López, C. Baydaroglu, M. Gradzielski, C.P.R. Hackenberger, U. Alexiev, O. Seitz
Date
2024
Identifier
DOI: 10.1039/D4CC00884G
Source(s)
Citation
Chem. Commun., 2024, 60, 4785-4788
Type
Text